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Antiviral agents have been successfully screened using embryonated chicken egg as media for both virus cultivation and inhibition assays [ 13, 33].
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Figure 5 shows that 65 active compounds were successfully screened using pharmacophore- and docking- based VS.
A total of 2520 positive colonies were successfully screened using PCR with primer-probes (AC 10/ AG 10.
These strategies resulted in 84.8% of children being successfully screened.
Recent improvements in PCR technology and protocols have made BAC screening more efficient and several genes have been successfully cloned using PCR to screen BAC libraries [ 19- 22].
Such screens have been successfully accomplished using zebrafish, leading to clinical trials (North et al., 2007; Goessling et al., 2011).
Single molecular haplotyping has also been successfully used to screen samples for specific inversion variants [ 23].
In addition, immunoassays based on conformational antibodies have been successfully used to screen DAACPs at the tissue and cellular level.
Libraries constructed with this method and targeting more than 10,000 different human genes have been successfully used for screening [ 3, 4].
The phage display technique first introduced by G. Smith 49 has been successfully used to screen a large peptide libraries that binds to a specific antibody.
Wnt3a-Gluc, a fusion protein comprising Wnt3a and Gaussia luciferase (Chen et al., 2009), has been shown to act as a functional ligand and has been successfully used to screen for small molecular inhibitors of Porcupine (Chen et al., 2009).
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CEO of Professional Science Editing for Scientists @ prosciediting.com