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Moreover, it has been successfully established, using microbes, that social behaviours often involve frequency-dependent selection (Queller 1984).
Purification of antibodies expressed in plants has been successfully established using protein A- or G-based affinity chromatography [ 77].
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Triticale was successfully established using no-till methods after termination of an alfalfa-grass field.
Therefore, the industrial production process of S-DMCPCA was successfully established using recombinant R-amidase from D. tsuruhatensis.
HCEC primary cultures were successfully established using 20% HAF-containing medium, and these cultures demonstrated rapid cell proliferation according to the cell proliferation and death ELISA assay results.
In conclusion, we demonstrated that immortalized porcine fibroblasts were successfully established using the human hTERT gene, and the TALENs enabled biallelic gene disruptions in these immortalized cells.
A hydrothermal process (steam autoclave) resulted in accelerated temperature-controlled reduction of AgNO3 in which AgNO3 reduction to metallic AgNPs with robust control over optical and structural properties was successfully established using steam autoclaving at 100 kPa and 121 °C for 20 min.
Thus, an effluc reporter gene was additionally adopted for tracking the migration of DCs and dual reporter DCs expressing both the NIS and effluc genes were successfully established using a viral vector system expressing the reporter genes.
Recently, culture systems for preantral follicles [ 19, 20] and primordial follicles isolated from newborn mice ovary [ 21] have also been successfully established and used to obtain live offspring.
A small scale evaluation has already been successfully established [ 23] and could be used as a basis for a nationwide study.
Methods for the generation of microarrays were successfully established when using antigens derived from membrane-based selection.
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