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Therefore, these approaches have been successfully established as routine methods, together with classical biochemical tests and genome sequencing.
In the setting of the critically ill patient in the intensive care unit, GEDVI has been successfully established as an independent marker of cardiac preload in perioperative patients [3] and patients in septic shock [4].
The budding yeast, Saccharomyces cerevisiae, has been successfully established as a model to study the mechanisms of aging.
Among these methods, PCR has been successfully established as a valuable method which offers the rapid, sensitive, and specific detection of the selected genes in various pathogens such as Escherichia coli O157 H7, Listeria monocytogenes, Salmonella spp., and Shigella spp. [ 7, 8].
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MRI guided breast biopsy has been successfully established at UHCW NHS Trust which currently serves as the regional referral centre in the West Midlands.
In short, a long-term WIRS model was successfully established as demonstrated by the series changes that were observed, including the behavioural, serum corticosterone, IFN-γ and IL-17 levels and gastric mucosal injury changes.
A total of 3 green algae, 1 diatom and 4 cyanobacteria were successfully established as unialgal cultures, and the majority assigned a species identification using a DNA barcoding approach.
Forty eight G418 resistant single clones were isolated with clonal cylinders and cultured in 24 well plates and thirteen of these independent clones as well as pooled clones were successfully established as cell lines designated as MCF-7-Vector, MCF-7-VDRff and MCF-7-VDRFF.
Human epithelial ovarian tumours were successfully established as xenografts in nude mice in 54% of cases.
Three low expressing K15ΔNLef1 founder lines were successfully established as detected by PCR (Lef1for 5′-TGTCCCTTGTATCACCATGGACC-3′ and Lef1rev 5′-CCAAAGATGACTTGATGTCGGCT-3′ and K15for, β-globrev) and Southern blot.
The pre-absorbed immunoproteomics method (PAIM) was successfully established as a useful tool for identifying bacterial surface proteins in our previous study [ 12, 13] and has been applied in Escherichia coli [ 14] and Campylobacter jejuni [ 15].
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