In our context, three Ni3S2 nanostructures directly grown on Ni foam were successfully designed using a facile hydrothermal method.
It could be concluded that a promising NAP-ECPs was successfully designed using QbD approach in a laboratory scale.
The in vitro release profile of enoxaparin sodium was close to the ideal one, therefore the system was successfully designed using QbD approach.
A sensitive and label-free electrochemiluminescence (ECL) aptasensor for the detection of adenosine triphosphate (ATP) was successfully designed using host guest recognition between a metallocyclodextrin complex, i.e., tris bipyridine)ruthenium II -β-cyclodextrin [truthenium II -β-cyclodextrin-binding aptameruthenium II -β-cyclodextrin
Based on those SSRs, 39,336 primer pairs were successfully designed using Primer3 (Additional file 12).
A total of eight primer/probe sets were successfully designed using the LightCycler® probe design (Roche Applied Science Germany) software 2 (Additional file 2: Table S2).
Further, 5,692 SSR primer pairs were successfully designed using software Primer 3. The details of the frequency of SSR motif and genic-SSR primers sequences (including designing parameters) are summarized in Additional file 4.
A total of 62,342 primer pairs flanking the SSRs were successfully designed using the public shareware Primer 3.0 (http://www-genome.wi.mit.edu/genome_software/other/primer3.html)., based on criteria of melting temperature, GC content and the lack of secondary structure.
The most abundant repeat type was (AG/CT), followed by (AAG/CTT), (ATC/ATG), (ACC/GGT), (AAC/GTT) and (AGG/CCT), respectively.. Based on the 5,883 SSRs, 3,919 primer pairs were successfully designed using Primer3 (Additional file 2: Table S2).
Further, 6,082 SSR primer pairs were successfully designed using Primer 3. The details of the frequency of SSR motif and genic-SSR primers sequences (including designing parameters) are summarized in Additional file 8. Very recently, we revealed that Chinese yam species have rich genetic diversity and phenotype traits including stable tuber yield, taste, texture, and dry matter content [ 28].
