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The possibility to benefit from deep sequencing data for quasispecies analysis has already been shown before with African horse sickness virus and classical swine fever virus [6], [12].

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This provides a broader perspective on why the results in Fig. 2 show higher antibody binding activity with TB pos patient sera than with TB neg sera as was shown before with larger numbers of human patient sera from the same collection (Schleicher et al., 2002; Thanyani et al., 2008).

Although the design of our experiments did not allow us to quantify fitness benefits it has been shown before that association with familiar individuals relaxes food competition and enhances reproduction compared with association with unfamiliar females (Strodl & Schausberger 2013).

However, it has been shown before that accessions with an active FLC allele differ in their vernalization requirement, with much of this variation mapping to FLC itself (S hindo et al. 2006).

"An early modern human population left Africa much earlier than had been shown before and met with Neanderthals, possibly those moving from Europe towards the East, some time around 100,000 years ago," said Sergi Castellano, who led the study at the Max Planck Institute for Evolutionary Anthropology in Leipzig, Germany.

It has been shown before that patients with ICA occlusion and a compromised flow state of the brain may improve spontaneously over time [25].

SDH has been shown before to be associated with changes in subsarcolemmal mitochondria with endurance training (Chilibeck et al. 1998, 2002) and as an element of the Krebs cycle and respiratory complex II (Fig.  1b), SDHA, is a critical element in mitochondrial metabolism.

It has been shown before that, in accordance with the 'posterior prevalence' phenomenon, Abd-B.r suppresses Abd-B.m (Casanova et al., 1986).

The stimulation of proteasome activity by 1.8-2.2 1.8-2.2 a biologically signifoldnt iscrease that has biologicallyefore by other natural compoundsignificanteasome actincreaseproperthat [26].

It has been shown before that P. falciparum parasites with a deletion of PfSERA4 gene display increased RNA levels of PfSERA5, which is refractory to gene deletion (McCoubrie et al., 2007).

Since it has been shown before that ES patients presenting with fever at diagnosis had a higher risk for metastases and death from their disease [ 9], and that IL6 is a main mediator of the febrile systemic response [ 42], we were intrigued to correlate IL6 serum levels with clinical data from ES patients.

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