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Low inertia and intrinsic stiffness have been set as additional design goals expressed by the minimal link length requirement.
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Data filtering was performed using percolator, resulting in 1% peptide false discovery rate (FDR), and Mascot peptide ion score > 20 was set as additional filter.
To prevent data from unusually large herds from influencing the study, an upper limit of 60 cows per herd was set as an additional inclusion criterion.
This was set as an additional percentage of the risk increase due to the first fracture (50% increase for GIOP patients and 100% for PMO patients).
Taking into account inter-individual differences in methylation levels and following Illumina Inc. recommendations, a 15% group-wise difference of methylation levels was set as a cutoff additional to PMann–Whitney<0.01.
The concentrations of proteins and kinetic parameters were set as described in Additional file 1 and 2. All signal proteins (B to F) were activated rapidly after the ligand addition under conditions in which all signal proteins were activated at a high level.
Statistical significance for TRE overrepresentation was set as p < 0.05 with additional filtering performed by setting the false discovery rate (FDR) at the level described (see Results section).
Taking into account the friction between the material and the steel baffle, the additional load was set as 20 kN.
In the current implementation, the sizes of these additional fields are set as follows: T s is 4 bytes, m is 4 bytes, c s is 32 bytes, P is 16 bytes, and c r is 32 bytes.
The phonation threshold is set as E=E thr=0.2 [sound examples in Additional files 5 and 6]8.
Additional plants were set as a border around the experiments in order to avoid edge effects.
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