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Several uncultured single microbial genomes have recently been sequenced using the single cell approach [15], [16], [17], [18].
Changes identified in the LSJ1 strain were compared to those in a total of seven N2-derived mutant lines that had been sequenced using the Illumina platform.
The chromosome A3 in B. rapa has been sequenced using the traditional Sanger technology.
The genome of an individual (NA19240) from the Yoruba in Ibadan, Nigeria has been sequenced using the 454 single-end method to ~5× sequence coverage.
Mitogenomes of seven sperm whales, including three that had been sequenced using the Illumina technology, were sequenced on a Roche GS Junior 454 (supplementary material S2, Supplementary Material online).
Many genomes have been sequenced using the whole-genome shotgun method, in which the sequence assembly is prepared from short, unmapped sequence reads with the help of specific software.
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Plasmid DNAs were sequenced using the 3730 DNA Sequencer (Applied Biosystems, Foster City, CA).
Libraries are sequenced using the Illumina MiSeq benchtop sequencer.
The cDNA libraries were sequenced using the Illumina HiSeq2000 platform.
Two RRBS libraries (X9015 and X9006) were sequenced using the Illumina GAII platform and the other libraries were sequenced using the Illumina HiSeq2000 platform.
The PCR product containing FUS with EGFP (Allele A) was sequenced using the reverse primer (CGGTGGTGCAGATGAACTT).
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