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In addition, neither of the var2csa-type sequences that have been sequenced in a multi-copy genome displays any unique sequence variation and they have the same polymorphic positions as all other var2csa-type sequences.
Using NGS technologies, a large number of ecotypes have been sequenced in a much shorter span of time [ 5].
The human sequence was a single long-range PCR product from a human Melanesian sample, the remainder of this mitochondrial genome having been sequenced in a previous experiment.
In the NCBI GenBank each of these genes have been sequenced in a number of isolates, varying from 48to6969.
VvNAC26 was chosen as a candidate gene that has been sequenced in a set of varieties to determine the existing nucleotide variation, and to identify its possible contribution to the natural variation observed for several reproductive traits in grapevine.
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All amplified fragments were sequenced in a ABI3730XL automated sequencer.
Additionally, TERTp was sequenced in a separate cohort of 19 patients with radiation-associated meningiomas.
The genomes of microbes can now be sequenced in a matter of weeks, giving new insights into their structure.
Increased sample throughput and clever barcoding strategies allow for several sample libraries to be sequenced in a single instrument run2,3,4,5,6.
For example, small and large RNAs from the same sample cannot be sequenced in a single sequence run.
The amplicons were sequenced in a 24-capillary 3500xl Genetic Analyzer (Applied Biosystems).
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