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The neurohypophysial hormone gene locus has not been sequenced from either cartilaginous fishes or jawless fishes.
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A small portion (<100 bp) of intron 1 in the amplified genomic fragment could not be sequenced from either direction after several attempts.
Seventeen BAC clones could not be sequenced from either end, whereas sequencing failed from the 5′-end for six and from the 3′-end for four.
Most detected peaks had similar numbers of tags from plus and minus strands, since double stranded DNA fragments are equally likely to be sequenced from either direction.
Various insert sizes, from 2 kb to 40 kb (fosmid) were sequenced from either end with read lengths of 200 600 bp.
Because paired end reads were intrinsically associated, we defined a 'fragment' as a cDNA fragment that was sequenced from either end.
In the ChIP-seq protocol with Illumina Genome Analyzer [ 14, 15], the ChIP-ed DNA fragments are sequenced from either end randomly.
The amplicon population resulting from the PCR products obtained is sequenced from either strand using primers containing a longer universal primer region (UP) at the 5', the RS, and the non polymorphic portion of the CP region at the 3'.
Multiple clones were sequenced from each isolation.
PTEN was sequenced from constitutional DNA from 25 families.
cDNA clones derived from dry seeds were sequenced either from 3'-ends using the M13 forward primer (5'-CCAGGGTTTTCCCAGTCACG) or from 5'-ends using the T7 primer (5'-TAATACGACTCACTATAGGG). cDNA clones obtained from the germinating seeds were sequenced from 5'-ends using the M13 reverse primer (5'-CGGATAACAATTTCACACAGG).
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