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The Littlest Genome The genome of the smallest single-celled algae, a bit of plankton just a few microns in diameter, has been sequenced by a team of researchers in France.
One is japonica cultivar Nipponbare, which has been sequenced by a BAC-by-BAC approach [ 24], and the other is indica cultivar 93-11, whash has been sequenced by a shotgun approach [ 25].
The bovine genome has recently been sequenced by a combined bacterial artificial chromosome and whole-genome shotgun approach [ 19].
Although the Jatropha genome has been sequenced by a combination of the conventional Sanger method and next-generation multiplex sequencing methods [ 36, 37], most of the molecular studies on Jatropha have focused on lipid metabolism in seeds.
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ITS2 and trnL amplicons were sequenced by a Roche 454 GS FLX Titanium sequencer with default parameters (XLR70 sequence kit, 2 × 200 cycles).
For CVM screening, the samples were sequenced by a Big Dye Terminator chemistry on an ABI 3100 Avant Automated DNA Sequencer (Applied Biosystems, Foster City, CA, USA).
The purified samples were sequenced by a Big dye terminator chemistry on an ABI 3100-Avant DNA sequencer (Applied Biosystems, Foster City, CA, U.S.A).
For many years DNA was sequenced by a method that was developed by Frederick Sanger in 1975 and used to sequence the first human genome in 2003, at a probable cost of at least $500 million.
The obtained amplicons were sequenced by a commercial sequencing company (Sangon Biotech, Shanghai, China).
Two partial metH sequences, one located near the 5′-terminus of the gene and the other near the 3′-terminus, were sequenced by a PCR based method.
The screened BAC clones were sequenced by a shotgun strategy.
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