A total amount of 17 Mb was sequenced allowing the discovery of 6,106 single nucleotide polymorphisms (SNPs).
The protein was sequenced, allowing the gene to be identified and cloned for production of recombinant protein in a follow-up paper [ 13], which characterised the reaction products of the enzyme acting on decorated xylotetraose, and classified the enzyme as belonging to the newly founded glycoside hydrolase family 115.
Using NGS, entire risk haplotypes, loci, or even entire genomes can be sequenced, allowing for the identification of all possible variants.
These clones were completely sequenced allowing the characterization of a total of 139420 bp, with the pgip region spanning about 50 Kb.
Aptamers were subcloned and sequenced, allowing the identification of a highly conserved consensus sequence, and showed a Kd value for l-Tyrosinamide of 45 μM.
The query primer is a mix of oligos that are degenerate for all positions except a single position that is being sequenced, which allows the sequencing of a single position based on the design of the query primer.
A large number of mtDNA amplicons were sequenced to allow the consensus sequence to be determined accurately, without laborious cloning and sequencing of PCR products.
SB2390 (cur15505, isolated in Curaçao, 2002, urinary tract infection; belongs to ST14) and SB3193 (IPEUC-744, isolated from a metritis case in a mare, 1981 in France; belonging to ST82) [ 23] are non-virulent strains that were sequenced to allow the comparison between virulent and non-virulent strains.
NGS technologies [e.g., Illumina/Solexa (Illumina Inc ., GA Roche/454 GS FLX Titanium (Roche Diagnostics Corp ., and ABI/SOLID (Applied Biosystems)] allow complete "miRnomes" to be sequenced and allow for the discovery of novel miRNAs and isoforms.
Because bacterial genomes are relatively small in size (typically 1 10 Mbp) they can be sequenced in multiplex, allowing the high read depths of e.g. a single lane of Illumina HiSeq to be distributed among up to 96 different samples via the use of individually-tagged libraries [ 4- 7].
