To test for rare genetic mutations, a cohort of patients with unexplained early-onset scoliosis (EOS) was screened using high-density microarray genotyping.
The yeast-displayed EETI-II libraries were pooled, and were screened using high-throughput fluorescent-activated cell sorting (FACS) to isolate mutants that were well-expressed on the yeast cell surface and bound with high affinity to detergent-solubilized αvβ3 integrin.
The coding and flanking intronic regions of PALB2 in DNA extracted from blood were screened using high-resolution melt curve analysis with Sanger sequencing confirmation.
Variant enzymes were screened, using high-throughput GC MS, for their ability to catalyse the formation of the double aldol adduct 14.
The catalytic oxygen electroreduction properties of platinum alloyed with base metals were screened using a high throughput combinatorial method.
In this first round of genotyping, 4,000 subjects are being screened using custom high-throughput 384-plex Illumina arrays (Illumina, Inc., San Diego, CA).
Thus, the 380-member CCa2X library was screened using an enzyme concentration 3-fold higher than that used for the earlier experiments.
An effective scoring function should produce promising drug candidates which can then be synthesized and physically screened using high throughput screening (HTS) process.
In more practical terms, an effective scoring function should produce promising drug candidates which can then be synthesized and physically screened using high throughput screening process.
Over the next 3 years, >30,000 clinical specimens from US patients with suspected tickborne diseases will be screened by using high-throughput molecular methods designed to detect bacteria, followed by use of genomic sequencing to characterize detected pathogens.
