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First, the derivatization procedure had been screened by experiments according to a complete factorial design in order to evaluate the statistically significant variables.
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Based on this purpose, a set of 5000 molecules from the Zinc database have been screened by docking experiments using AutoDock Vina software.
With the aim to identify new chemical entities to be used for further rational drug design, a set of 3000 molecules from the Zinc Database have been screened by docking experiments using AutoDock Vina software.
The influence of additions of LiOH, NaOH and KOH on the activity was screened by pulse experiments carried out in the absence of gaseous oxygen; FTIR reflectance spectra, NH3 adsorption data and some TPR data were also obtained.
Various commonly proposed inhibitors were screened by static etch rate experiments and only BTA and uric acid were found to be effective in the alkaline pH range, while potassium sorbate was moderately effective.
Then, the vasodilator effects of theses compounds were screened by rat aortic ring experiment.
For the wild type and ΔthdF mutant S. Typhimurium LT2 recipient strains, one hundred independent transconjugants of three different mating experiments were screened by PCR on the left junction for SGI1 integration (1st and 2nd attB sites) and by PCR using primers SGI1circ1 and SGI1circ2 oriented towards the left and right end of SGI1 for tandem integration (Table 1, Fig. 1).
All mice used in every experiment were screened by PCR prior to their use in subsequent assays.
Individual sera from three rats were screened by Western blotting, and the serum with the strongest specific signal was used in further experiments.
The slides were screened by one pathologist.
Next, all samples were screened by CLSM.
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