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The structure of the protein-channel complex has been resolved by electron and X-ray crystallography.
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For instance, a radical species can be resolved by electron paramagnetic resonance spectroscopy (EPR), while its spectroscopic signature in time-resolved visible or infrared spectroscopy measurements may be more difficult or even impossible to assign.
This can be resolved by electron tomography, which involves tilting a relatively thick (for example, 300 nm) section and obtaining images at different angles relative to the specimen.
DOI: http://dx.doi.org/10.7554/eLife.01319.003 The structure of the AKAP18γ PKAholo complex was resolved by electron microscopy of negatively stained particles since these complexes are too small to be imaged in vitrified ice.
For some viruses, the structure of the most important immunogen, the envelope protein E, has been determined to atomic resolution by X-ray crystallography, and the architecture of virus particles has been resolved by cryo-electron microscopy.
This issue has been resolved by vendor.
Endocytic structures that can be defined morphologically include macropinosomes, which can readily be resolved by light microscopy (Liberali et al., 2008), and caveolae which are distinctive in electron micrographs (Parton and del Pozo, 2013).
The electron transfer reactions in cytochrome oxidase are very rapid, and cannot be resolved by stopped-flow mixing techniques.
The SCM contrast was clear; however, comparison of SCM images with secondary electron microscopy and AFM images shows that the quantum dots cannot be resolved by SCM [7].
Discrepancies were resolved by consensus.
Disagreements were resolved by a third reviewer.
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