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For monoclonal antibodies, the primary antibodies had been replaced with normal rabbit nonimmune IgG.
In the negative controls in which the primary antibodies had been replaced with normal rabbit IgG and normal mouse IgG or omitted, signals were scarcely observed (data not shown).
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Within weeks, the scaffolding dissolved and was replaced with normal connective tissue.
And when it was replaced with normal food, they ate less normal food, approximately 30% less by week 5.
As a negative control, the primary antibody was replaced with normal immunoglobulin.
4 8 hours after transfection the medium was replaced with normal growth medium.
Negative controls were treated with similar procedure except that primary antibody was replaced with normal rabbit sera.
Cells were visualized for 30 min during MiTMAB treatment, then the medium was replaced with normal imaging medium (washout).
At time 0, low calcium media was replaced with normal Ca++ (1.8 mM) media to induce cell-cell adhesion.
To induce differentiation, the medium was replaced with normal basic differentiation medium two days post-transduction and subsequently changed every other day until fixation.
Six hours after transfection medium containing transfection reagent and plasmid was replaced with normal growth medium and further incubated for 16 hr.
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