Organs and plasma are removed to evaluate inflammation and oxidative stress by q-RT-PCR, insulinemia by ELISA test and insulin pathway by Western blot.
Mice were monitored daily for neurological impairment at which time they were killed and brains were removed to evaluate for tumor development.
At the end of the collection period for hepatic bile the intestine was removed to evaluate contractile responses of smooth muscle [ 9].
The animals were sacrificed at 2, 4, and 8 weeks; the surrounding tissue along with the implant was removed to evaluate biocompatibility and biodegradability by histologic analysis and GPC, respectively.
After mouse euthanasia, esophagus, stomach and cecum were aseptically removed to evaluate C. albicans colonization.
Since insulin injections prevent an accurate measure of endogenous insulin and glucose levels, the 2 OB/D subjects treated with insulin were removed prior to evaluating insulin resistance with the HOMA.
The trigeminal ganglion (TG) and cervical spinal cord (CSC) ipsilateral to the formalin injection and medulla-pons in toto of each animal were removed and processed to evaluate expression levels of the genes encoding for c-Fos (c-fos), TRPA1 (Trpa1), CGRP (Calca) and SP (PPT-A).
We selected barcodes containing as few "G"s as possible in order to avoid barcode biases caused by strand invasion [ 9], and pooled six different libraries to increase sequence diversity and address the low mappability observed in experiment 2. The linker was removed in order to evaluate whether using Illumina-recommended barcodes would improve read mappability.
Four days after immunization, a superficial lymph node (LN) was removed by surgery to evaluate the number of Ag-specific CD8+ T cells generated.
Thoracic and abdominal aortas were removed in order to evaluate VC, by von Kossa staining and calcium content quantification, respectively.
