NIRS may be utilized along with a vascular occlusion test, in which limb blood flow is temporarily occluded and released, to quantify a tissue bed's rate of oxygen exchange during ischemia and recovery.
Upon binding of C5a to the receptor, N-acetyl-β-D-glucosaminidase (NAG) is released, which is quantified by addition of its substrate.
The release behaviour of insulin from the nanoparticles has been studied to quantify released insulin and folic acid with time using high performance liquid chromatography.
A homogeneous erodible soil depth of 1.5 m was selected since there was no detailed soil depth map available (this depth was also assumed to quantify the released initial volume).
A colorimetric assay kit was used to quantify the LDH released from the cultured NSCs.
In addition, time resolved, non-invasive measurements were used to quantify Ag+ release rate (efflux) from two common antimicrobial biomedical devices, and Ag+ uptake rate (influx) to a mature Pseudomonas aeruginosa biofilm during disinfection.
Pharmingen's OPTEIA IL-10, IL-4 and IFNγ kits (Pharmingen, San Diego, USA) and the TGFβ1 Emax® ImmunoAssay System (Promega, Madison, USA) were used to quantify cytokine release to culture supernatants, as previously described [6].
ELISA was used to quantify the release of three molecules with potential neuroprotective activity in the brain or retina: IGF-1 [50] [52], FGF-2 [53], [54], [54] and GDNF [11], [22] [30].
At Days 4, 8, 12 and 16, conditioned media were collected to quantify the release of newly synthesised PG.
Western blotting was used to quantify the release of selected proteins in the presence or absence of the cyclooxygenase-2 specific nonsteroidal pro-inflammatory drug carprofen.
