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Core components in ABA signaling, including OsPYLs, OsPP2C, OsSAPK2, and OsOREB1, have been identified in rice, and the ABA signaling transduction pathway has also been reconstituted in a protoplast system (Kim et al. 2012).
These findings clearly indicate that the observed folding of TtoA was the result of the activity of TtOmp85 and that purified TtOmp85 had been reconstituted in a functional manner.
ESM Fig. S 1 shows an example of one such design in which conductance is measured for ion channels formed by gramicidin that have been reconstituted in a lipid bilayer supported over an ~70-nm diameter nanopore in a silicon chip.
Another delicious variation calls for fresh blueberries and some sultanas (white raisins) along with diced, dried apricots that have been reconstituted in a little brandy, port or hot water; add these at the last minute of the cooking stage.
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This "bypass" pathway has been reconstituted in an in vitro isolated WD culture system and reliable GDNF/Ret-independent budding has been achieved with the exogenous addition of an FGF (i.e. FGF1 or FGF7) together with simultaneous inhibition of activin signaling with follistatin (Fig. 1) (Maeshima et al., 2007; Rosines et al., 2007; Choi et al., 2009; Tee et al., 2010).
The humble hooded anorak – the surprise standout trend from the month of shows – was reconstituted in a mashup of technical and luxurious fabrics.
In any democracy, this would be hugely problematic so the bar for such a decision should be set exceptionally high.The fallout at home would be serious, even if the AKP were reconstituted in a different form.
Protein extracts were reconstituted in a sample buffer [0.062 M Tris HCl, 2% sodium dodecyl sulfate (SDS), 10% glycerol, and 5% β-mercaptoethanol].
This was repeated at least one more time, after which the sample was reconstituted in a known volume of 0.15 moles per liter (M) nitric acid (trace metal grade).
When these pathways are reconstituted in a heterologous microbial host for metabolic engineering purposes, the acyl-CoAs may be subject to undesirable hydrolysis by the host's native thioesterases, resulting in a waste of cellular energy and decreased intermediate availability, thus impairing bioconversion efficiency.
We embarked on an examination to see whether a complete functional bacterial luciferase pathway can be reconstituted in a transplastomic plant to produce both the luciferase and luciferins.
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