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Rhodotorula (Rhodosporidium) toruloides is a yeast that naturally synthesizes substantial amounts of specialty chemicals and has been recently engineered to (i) enhance its natural production of lipids and carotenoids, and (ii) produce novel industrially relevant compounds.
On the basis of the finding that transfer of hematopoetic stem cells expressing major allergens can induce robust allergen-specific tolerance in a preventive model of allergy [ 72,73], membrane-anchored and secreted allergen-expressing stem cells have been recently engineered to delineate the mechanisms of tolerance [ 74].
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Here we present a mathematical characterization of a novel fluorescent reporter that was recently engineered to have a short half-life (∼12 min).
P. furiosus was recently engineered to generate 3-hydroxypropionate (3-HP) from CO2 and acetyl-CoA by the heterologous-expression of three enzymes from the CO2 fixation cycle of the thermoacidophilic archaeon Metallosphaera sedula using a thermally-triggered induction system.
In vitro mimics of cellular machines have been recently engineered and utilized to investigate processes within cells.
"Single-shot" ablation devices have been recently engineered.
This may soon change as a new generation of ektacytometer has been recently engineered.
In addition to their applications in genome editing and gene expression regulation, programmable DNA recognition systems, including both CRISPR and TALE, have been recently engineered for the visualization of endogenous genomic elements in living cells.
For each control point, we discuss the main natural classes and mechanisms of RNA-based gene regulation and those that have been recently engineered by synthetic biologists (Fig. 2A and B).
Toluene-4-monooxygenase from Pseudomonas mendocina KR1 was recently engineered for the synthesis of hydroxytyrosol, a potent antioxidant.
Numerous near-infrared (NIR) fluorescent proteins (FPs) were recently engineered from bacterial photoreceptors but lack of their systematic comparison makes researcher's choice rather difficult.
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