The factor V Leiden mutation test should be performed using any of the accepted technical approaches as long as they have been properly validated by the laboratory, while adhering to current ACMG/CAP quality assurance guidelines for molecular genetic testing.
Therefore, the two RT-qPCR methods for the analysis of the relative expression of the otapks and otanps genes have been properly validated and can be used as control tools to avoid or minimise the presence of OTA in ham.
Nevertheless, diagnostic accuracy for both exacerbation presence as well as severity categories has never been properly validated.
10 11 However, none of them have been properly validated.
None of the currently used clinical severity indices for patients with IBD have been properly validated.
'Pulmonary infection scores' have not been properly validated, and suggesting the use of them is not consistent with current evidence.
None of the currently used clinical severity indices for patients with inflammatory bowel disease (IBD) have been properly validated.
Nevertheless, any putative marker identified in this study has to be properly validated in a larger cohort, as it is likely that the full extent of variability in this type of critical care population has been under sampled.
We reiterate that it is critically important that ChIP antibodies be properly validated; tied to this, it is essential that the validations are properly reported on companies' antibody information sheets, and in research publications.
Whenever regression models are optimised, it is important that all optimisation steps are properly validated.
