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The cells have been propagated in vitro for 5 years and showed 100% aneuploidy and a mixed epithelial-fibroblastic morphology.
Cell lines of 17 breast cancer specimens had been established and the cells had been propagated in vitro for a median period of 2 years.
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These cells could be propagated in vitro and potentially induced to acquire glucose-stimulated insulin release (GSIR) ability.
Recent studies have shown that NSCs isolated from mammalian CNS including human can be propagated in vitro and then implanted into the brain of animal models of human neurological disorders.
Commercially, plants may be propagated in vitro and then planted out to grow into plants large enough to sell.
Mouse PGCs can be propagated in vitro for short periods before undergoing apoptosis [55].
One of these metastases was propagated in vitro (K5L) and further analyzed.
Neurospheres were propagated in vitro and assayed for self-renewal and cell differentiation after 4 in vitro passages (IVP), as previously described [46].
Furthermore, dissociated YFP+ cells from tertiary neurospheres were propagated in vitro for more than 10 IVPs, maintaining features of neural stem cell cultures (not shown).
It is however possible that some mutations such as 769N have a heavy fitness cost, as parasites harbouring the mutation could not be propagated in vitro under standard culture conditions.
For feeder-free culture of iPS cells, the plate was coated with gelatin (Stem cell Technology, INC .. Mouse ESCs (J1) were propagated in vitro using feeder-free conditions without signal inhibitor supplementation.
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