Loss array elements were assigned a value of −1, retained elements a value of 0, and gained elements a value of 1. Twenty CIS specimens were profiled in total, and a threshold for gene-specific copy number gain/loss was set at 20%.
Targeted metabolites were profiled to confirm putative changes in specific metabolic pathways.
In each case, there is specificity: specific markers, specific genes, specific molecules specific antibodies, specific targets.
However, the difference may be explicable in terms of different experimental conditions: in the previous study, expression profiling was performed in specific cell type (i.e. T cells), whereas PBMCs, as used here, include various cell subtypes.
Onto-Express [ 22] was used to determine whether clusters of genes with similar expression profiles were enriched in specific GO functional categories.
Additionally, the miR-17-92 miR-17-92 miR-17-92patients, whiclusterbeen involved in sprofile types of lympatients and lymphocytic disorder, whichownregulated [ 41].
It is believed that B-cell lymphoma subtypes show characteristic gene expression profiles of B-cells that are arrested in specific developmental stages [ 57].
10 An increasing number of studies have suggested deregulation of lncRNAs in cancers, 9 11 12 and reports on lncRNA expression profiles in specific cancers are beginning to be published.
To test these hypotheses, a linear model was employed to compare expression profiles in specific tissues of both mouse strains at baseline (day 0) and between baseline and post-infection (days 3 and 6 versus day 0).
Several methods are used to characterize the miRNA expression profiles in specific tissues such as Northern blotting, RNase protection assay, RT-PCR and microarray analyses.
