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This commonly used methodology has been previously validated using technecium-99 single-photon emission computed tomography in concert (not used in this study) [ 14].
Methods used for DNA isolation, amplicon library preparation, sequencing and microbial profile analysis have been previously validated using a mock community of oral microorganisms [ 20].
DOI: http://dx.doi.org/10.7554/eLife.05604.025 Since the genes underlying variation in the aliphatic GSL pathway investigated in this study have been previously validated using lab techniques, we have a solid working knowledge of the resulting laboratory GSL profiles (Beekwilder et al., 2008; Hansen et al., 2008).
The PHQ was used in this study as it has been previously validated using nominal techniques [ 34] and utilised in Sri Lanka for a number of epidemiological studies (National Mental Health Survey with a sample of 6000 participants, Colombo Twin and Singleton Study with a sample size of 6000, a study measuring CMD among displaced populations with a 450 sample) [ 35- 37].
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The finite element model is previously validated using experimental results for the transient response, mode shapes and transmissibility functions.
A constraint-based model of P. pastoris was previously validated using metabolic flux analysis (MFA).
The laboratory was previously validated using the University of Guelph (Pearson correlation r = 0.91, n = 200).
This questionnaire, indexing bimanual usage, was previously validated using limb acceleration data, collected in ecological settings (Makin et al., 2013a).
These "security" algorithms were previously validated using analyses of discordances versus liver biopsy [ 7] and discordances with liver stiffness measurements [ 8, 9].
The use of a population plasma activity curve for yohimbine data based on the absence of peripheral metabolism of yohimbine in swine [ 12] was previously validated using these 2 anesthetics during early tracer development measuring arterial input and protein binding.
The Amharic translation used has been previously validated and used in Ethiopia mainly in relation to post-conflict displacement [ 37, 38, 42, 43].
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