Sentence examples for been previously typed using from inspiring English sources
The 300 MHC class I alleles of these 50 individuals have been previously typed using the PCR-SSOP method [ 16].
As a proof of concept, we applied the method to the recently released 37-nucleotide paired-end RNA-Seq data of 50 Utah residents with ancestry from northern and western Europe (CEU) [ 15] who are part of the HapMap project and have been previously typed using the PCR-SSOP method [ 16].
Genotyped DNA samples included a subset of the samples that had previously been typed using the VNTR LEI0258 [ 46].
The SCCmec elements were typed using previously described PCR primers and conditions and by array hybridisation as described below.
The Y Alu insertion (YAP), RPS4Y711 (C to T substitution), M9 (C to G substitution), M175 (−5 bp), M95 (C to T substitution), SRY+465 (C to T substitution), DXYS5Y (G to C substitution), and LINE1 insertion were typed using the previously described protocol [20].
The SCC mec cassettes were typed using a previously described multiplex PCR-method [ 32] with the following modifications: In M-PCR-1 primers γR and γF had a final concentration of 0.3 µM, while all other primers in M-PCR-1 and M-PCR-2 had a final concentration of 0.2 µM.
The Schu S4 strain used in this study was typed using PFGE as previously described [ 11, 14].
The rate equation derived from this scheme is: The kinetic parameters were fitted with the equation and are presented in table 1. Kinetic constants for the wild type enzyme have been previously estimated using the effects of a substrate analogue on substrate hydrolysis and on decarbamoylation [ 18].
In fact, this type of approach has been previously used by us with Luciferase reporters, where it is easier to control these effects (See Sanford et al. (1994) Genes Dev.; Michlewski et al. (2008) Mol Cell).
The results of this analysis were compared with variation observed in two cell-surface components that have been previously used for bacterial typing: the heat-stable (HS) serotyping antigen, which was investigated serologically; and the flagella, which was investigated by nucleotide sequencing of the flaA gene short variable region (SVR).
Computational screens of this type have not been previously used to ensure such a "lock-and-key" type relationship between cdODN sequence and disease phenotype.
