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We focused the analysis on the NATA2 locus, which had not been previously targeted using engineered nucleases.
To test whether our ZEN protocol is applicable to other genes, we used electroporation to target 10 genes that have been previously targeted using microinjection.
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He has also been previously targeted by spyware.
This has resulted from the likely reduction in opportunities for rebirthing/cloning through the vehicle registration system which was previously targeted by a potentially large number of used vehicle dealerships and private sellers attempting to sell stolen vehicles.
Although we previously showed that CLL may be targeted using IP3R-derived peptides, we now provide (i) evidence that this strategy is applicable in other cancer cells like DL-BCL, and (ii) mechanistic insights in the underlying signaling pathways revealing a prominent role for IP3R2.
The Wnt1cre transgenic mouse line has been previously used in numerous studies for targeted gene deletion and lineage tracing of NCCs.
Both cultured retinal explants and the I-R model have been previously used to evaluate neuroprotective strategies targeting retinal neurons, specially RGCs (Lagreze et al., 1998; Goodyear and Levin, 2008; Peng et al., 2011; Zhang et al., 2012).
Furthermore, GroEL has been previously used as a major target dominantly recognized by the immune system of infants vaccinated with WCV [44].
Nevertheless, this strategy has been previously used to identify the targets of BCR-ABL kinase inhibitor INNO-406 in chronic myeloid leukemia [ 30].
In addition, several agents belonging to PCR group II through IV (most of the PCR group I targets were previously tested using genomic nucleic acid templates at NRL) were also tested.
Some of the comprehensive studies performed on selected immunoproteomic targets were previously performed using molecular approaches.
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