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Since O. barthii has not been previously sequenced, we assessed the quality of our assembly via two independent approaches.
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Four of the seven samples have been previously sequenced using different sequencing technologies, and we denote these samples with an asterisk.
We obtained data from a cDNA library that had been previously sequenced by conventional Sanger (dideoxy-based) sequencing to identify SSRs [ 21].
Every patient had their genome sequenced, even those whose genomes had been previously sequenced.
Previous studies tested experimentally the accuracy in re-sequencing pools of strains of highly isogenic D. melanogaster, whose genome had been previously sequenced individually.
Strains representing these serotypes have not been previously sequenced.
An asterisk denotes samples that have been previously sequenced.
For two of the genes (At1g60987 and At2g02490) no ESTs have been previously sequenced.
Acinetobacter baylyi ADP1 has been previously sequenced and annotated (Barbe et al. 2004).
All endosymbiont genes analyzed here have been previously sequenced [ 25]; gene identifiers are available from Additional file 23.
The O139 serogroup isolate from Bangladesh (DB_2002) was collected in Dhaka in 2002 and has not been previously sequenced.
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