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The genes associated with EOAD (PSEN1, PSEN2 and APP) have been previously sequenced in all early onset cases (88 cases, with an age at onset before 65 years old) and no mutations were found [26].
Therefore, our transcriptome sequencing identified more than 8000 novel unigenes in the fruit and seed transcriptome that had not been previously sequenced in the species.
Identification of the TRDD genes within the bovine genome allowed us to evaluate TRDD gene usage in cDNA sequences that had been previously sequenced in our laboratory.
Other genes have not been previously sequenced in sugarcane, among them genes involved in the metabolic pathways not traditionally considered in sugarcane breeding, such as the carotenoid biosynthesis pathway.
This was accomplished by obtaining new sequences for four nuclear loci, thus significantly increasing taxonomic sampling, and by filling in gaps in sequence data from mitochondrial loci that had been previously sequenced in megabats by collecting new data from the additional taxa available to us.
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CYLD was previously sequenced in affected individuals from the Abalkhail et al. [ 1] ALS pedigree but no mutations were identified (Prof Jacqueline de Belleroche, personal communication).
The genomes of A. pacifica SW150T, A. megaterium XH134T and A. macrocephali JAMB N27T were previously sequenced in the lab, in which the first two strains were also isolated from SPG [ 20, 21] (Table 1).
We then screened for KRAS mutations in 30 non-small cell lung cancer biopsies that had been previously sequenced for mutations in EGFR exons 18 21.
A portion of the MAT1-1 idiomorph for A. nomius NRRL 13137 had been previously sequenced and accessioned in GenBank (HQ001936).
We then performed a blinded assessment on ovarian tumour DNA samples that had been previously sequenced for mutations in TP53 to assess the sensitivity and positive predictive value of the HRM technique.
SARS-CoV 2003VA2774 (an isolate from a SARS patient in Singapore), which has been previously sequenced (14 ), was propagated in Vero E6 cells.
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