For the assessment of our validation scheme, a subset of 800 genes is used which was previously selected using a periodicity test [20] and was first studied by Rhein and Strimmer for gene network inference [1].
However, M. hominis and M. pneumoniae cells with A2062G and A2062T mutations in the 23S rRNA gene have been previously selected in vitro using josamycin and shown to cause high-level josamycin resistance [ 33, 34].
The subclass specific reagents used had been previously selected on the basis of low cross reactivities among themselves, and ability to faithfully react with African heavy chains dominant allotypes [15].
To our knowledge, the only plant species previously selected using this dapA selection system was potato [ 25].
Among the 17 Beijing strains 8 (NHM77 HM903 HM764, HM764, 990172, W4, N4 and CAM22) from Europe were previously selected as representative of this genotype using several typing methods.
MS/MS settings used a trigger threshold of 8,000 counts, monoisotopic precursor selection (MIPS), and rejection of parent ions that had unassigned charge states, were previously identified as contaminants on blank gradient runs, or were previously selected for MS/MS (data dependent acquisition using a dynamic exclusion for 150% of the observed chromatographic peak width).
The CH1 and CL domain of the Fab fragment were replaced with a heterodimeric coiled coil (WinZip-A2B1), which had previously been selected using a protein-fragment complementation assay in Escherichia coli.
The first step mutant frequency of reference strains of C. albicans ATCC 90028, C. tropicalis ATCC 750, C. glabrata ATCC 90030, C. parapsilosis ATCC 22019, A. flavus MTCC 2799 and A. fumigatus MTCC 1811 were selected, using previously described method [ 22].
Adaptors were ligated and DNA was size selected using Agencourt Ampure XP beads (Beckman Coulter), as previously described [ 27].
HEK293T cells were transfected with the chicken FANCD2 constructs previously described (45), and clones were selected using Zeocin.
