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Although the use of intracardiac echography for fetal heart rate monitoring has not been previously reported, we used it because monitoring fetal heart rate with sterile precautions was necessary during CPB.
Even though proviruses related to Microviridae have not been previously reported, we set out to verify this possibility by performing searches against genomic sequences available in public databases.
In addition to miRNAs that have been previously reported, we also evaluated the predicted targets of the most differentially expressed miRNAs in our data with known or putative associations with oligodendrocyte differentiation and myelin production.
And because no optimum conditions for de novo synthesis of GC-rich genes have been previously reported, we compared both conventional methods of assembly, PCA and LCR in the presence and absence of DMSO and betaine during the construction of IGF2R and BRAF.
In addition, although a correlation between S303 and S307 phosphorylation and HSF1 protein stability has not been previously reported, we repeatedly observed two to three-fold higher steady state levels of HSF1 when the S303A, S307A and S303/307A mutants were expressed in yeast (Figure 1D, F).
Also, in an observation that has not been previously reported, we found that population-based rates of critical illness were systematically higher for people in lower income strata.
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As was previously reported, we found that multiple independent mutations in sei lead to rapid seizures and paralysis in response to acute heat stress.
Consistent with what was previously reported, we observed that autophagic flux increased over the first 3 to 8 d after doxycycline induction of HRASG12V as measured by LC3-II formation in the presence of CQ or BafA1 (Fig. 3A; Fig. S7A).
In this study, we report five mutations in the ABCA4 gene, two of which have not been previously reported, and we determine the clinical phenotype associated with these genotypes in five unrelated patients with STGD.
As had been previously reported [26], we found no correlation between the basal sublingual PVD and the ΔT°.
Detailed methods for most studies have been previously reported, and we therefore summarize only key aspects of each study's methodology here (Table 1).
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