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Temporal oscillations in phase have been previously quantified using OCT [ 33].
Bone erosions in mice with CIA have not been previously quantified using 3D reconstruction with micro-CT.
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Global baseline gene expression in liver on these samples was previously quantified using the Agilent 4x44 array [ 17] (see Methods for details).
The Ni tolerance of TgSATm was previously quantified using a metal growth, zone of inhibition assay, on plates, [ 19] (Fig 4A Ni).
This phenotype was previously quantified using a classic six-chambered countercurrent device first introduced more than 40 years ago (Akai, 1979; Benzer, 1967).
These emissions have not been previously quantified.
This is critical in light of the fact that stress levels in vegetation have previously been quantified using hyperspectral sensors, which are more expensive and do not cover large areas as compared to SumbandilaSat satellite.
Veterinary antibiotics have been previously identified and quantified using high-performance liquid chromatography with tandem mass spectrometry (LC-MS/MS) (Aust et al. 2008; Kim and Carlson 2007).
Plasma haptoglobin concentration was determined as the haemoglobin binding capacity using an appropriate assay (TP801: Tridelta Development Ltd., Greystones, Ireland), which was previously validated [ 32] and was quantified using a spACE automated analyser (Alfa Wassermann, Inc., West Caldwell, NJ, USA) [ 33].
The synaptophysin antibody (Sigma S5768; mouse synaptosome preparation from rat retina -1 500 dilution) has also been extensively tested and its immunoreactivity has been quantified using the same method previously reported [ 54].
UV detection was employed and the amino acids were quantified using previously determined calibration curves.
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