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The surface nanostructures have been previously produced using various nanofabrication methods, such as colloidal assembly [33], a sol gel technique [34], capillary lithography [35], electron beam lithography [36], pattern transfer of natural surfaces by plasma etching [37], and electrospinning [38].
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GO was previously produced using natural graphite powder (purum powder ≤ 0.1 mm, Fluka, Sigma-Aldrich Corp. St . Louis MO, USA) according to the Brödie method [13].
A genetic map that had been previously produced [ 51] was used for the QTL analysis.
No written guidelines had been previously produced.
Moreover, the size range obtained is much smaller than that previously produced using standard milling devices.
The populations used for QTL mapping were previously produced (Zhang et al. 2011).
The normal mortar describes previously is produced using a mixer HOBART, in a tank of five liters spreading with the characteristics of standard NFP 15-411 (NF P 1996).
Most of the gene-expressed RNAi libraries used for target identification and validation have been produced using computer algorithms and previously known genes or gene families.
For the current study, a ChAd63 vector expressing the same PfRH5 transgene was produced using previously described methods (Goodman et al., 2010).
Tissue microarrays (TMAs) for SKOV3 (four replicates) and primary tumour (two cores each) xenografts were produced using previously described techniques (Kononen et al, 1998).
To generate the former, the canonical HIV strain NL4-3 [22] was produced using our previously published transfection method [23], and virus was collected after a propagation step.
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