Sentence examples for been previously produced and from inspiring English sources
Transgenic tobacco plants (a natural host of Potato Virus Y, PVY) which constitutively express PVY-derived HC-Pro, have been previously produced and characterized in our laboratory [ 10].
Transgenic Nicotiana tabacum lines expressing the VSRS P25, HC-Pro and AC2 have been previously produced and characterized in our laboratory [ 34- 36].
P25 VSRS –expressing transgenic tobacco plants (Nicotiana tabacum nn), along with tobacco lines expressing various other VSRS (e.g. Potato virus Y potyvirus specific HC-Pro and African cassava mosaic geminivirus specific AC2) have been previously produced and characterized in our laboratory [ 34].
To quantify proteins expressed by E. coli (as described in 2.6), enzyme-linked immunosorbent assays (ELISA) were performed using the polyclonal rabbit anti-Cry8Ka1 antibody, which was previously produced and purified by our team (not shown).
VLPs of parvovirus B19 (B19V), have been previously produced in vitro and its stability and ability to assemble into hybrid particles composed of wild-type and chimeric proteins evidenced their potential as research tools.
VLPs containing biologically active glycoproteins from different influenza subtypes have been previously produced in insect cells and have been shown to elicit strong immune responses conferring protection against homologous or related heterologous viruses [18], [21], [22].
Vanillin has been previously produced from alkaline treatment and nitrobenzene oxidation of kraft lignin [ 31].
For example, full-length E1 containing the catalytic domain, linker and carbohydrate binding domain has been previously produced in transgenic tobacco plants.
By Wednesday, he was telling an audience in the Ruhr: "You can't distribute what has not been previously produced". So is Germany at last about to change?
