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The former provides evidence that transcription has been previously observed at a given location, while the latter arrays feature many probesets targeting both EST predictions and those arising from in silico methods such as Genscan [46].
Such Dgcr8-dependent, Dicer-independent reads have been previously observed at miRNA loci in Drosophila and mouse small RNA sequencing studies and appear to be a 5' remnant of Drosha cleavage that is further degraded by an unknown 5'-3' exonuclease [10], [19].
For example, mosaic patterns of gene conservation have been previously observed at a number of hyper-variable loci (LOS: [ 24, 25]; CPS: [ 26]; and RM: [ 27]).
Interestingly, although HP1 proteins themselves have not been previously observed at the centrosome/spindle pole, several HP1-interacting proteins are known to reside in this cell compartment.
We find evidence that gene expression levels, when compared between pure species, follow a pattern of greater conservation in the earlier stages, as has been previously observed at the level of gene sequence and morphology.
Lignifications and callused deposition have been previously observed at the interface of host plant cells with invading Orobanche haustorium [ 28] and is thought to be correlated with levels of host resistance to Orobanche.
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However, since comparable amplification levels (factors of 35) were previously observed at another rock slope instability (Preonzo, Burjánek et al. 2017), we suggest that this phenomenon might be not rare for instabilities close to collapse.
At the sand texture site with low initial pools of C and nutrients, FFR resulted in soil C and Ca reductions over the 20-year period, which may have caused large reductions in aspen growth that were previously observed at that site.
In the case of free CP Sso, optimum stability temperature might be at, or close to 75°C, as significant inactivation was previously observed at 85°C [ 33].
Our results show that the praziquantel-induced boost in worm-specific immune responses that was previously observed at six weeks after treatment [ 25, 26] lasts at least up to the time of delivery.
As was previously observed, at 350 °C the initial conversion was moderate (54 %) and deactivation was slow, giving 20%% conversion after 50 h on stream, whereas at 500 °C the conversion was initially very high (97 %) but declined rapidly after 8 h on stream.
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