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As a benchmark for our UCE discovery pipeline, we examined how many UCEs that had been previously identified we were able to recover.
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Although MAPKs from these plant species are previously identified, we have included them here to broaden the study.
The Ad-apr-1 cDNA sequence from A. duodenale had not been previously identified, so we amplified it from a cDNA library derived from mRNA from A. duodenale infective larvae using PCR and oligonucleotide primers complimentary to the 5′ and 3′ untranslated regions of Ay-apr-1.
To determine whether we could identify additional targets that had not been previously identified by MAS5, we applied an alternative method using the PLIER probe summarization algorithm to obtain signal values.
Since TEs have not been previously identified in S. japonicum, we conducted TE prediction by scanning the genome sequence using REPET software.
Furthermore, we were able to identify 23 gene clusters with similarities to known RiPP biosynthetic gene clusters, but that have not been previously identified in anaerobes and we were able to confirm a further 15 previously identified RiPP gene clusters.
6-hydroxy-6-methyl-3-heptanone has also been previously identified in mouse urine although we can find no report of this compound in human urine.
While CRKL (CRK-like) and FER non-receptor tyrosine kinase have been previously identified using high-throughoutput screening, we demonstrate here that these two kinases work together to control the EGFR signaling pathway.
We defined recovery as scoring 10 points or more, having been previously identified as cognitively impaired.
Celiac ganglia have not been previously identified by EUS.
This protein has not been previously identified in mammalian milk and its function is unknown.
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