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In ES cells, PcG complex binding has been previously identified through genome-wide location analysis and found to associate with, and transcriptionally repress developmental regulatory genes [16], [18].
Such an approach has also provided new insights into specific systems that have not been previously identified through hypothesis-directed research [17].
Altogether, 137 CWPs were identified in this study among which 51 had not been previously identified through cell wall proteomics.
In addition to the expected roles for protein chaperones and other cell-autonomous mechanisms that have been previously identified through cell- or biochemistry-based studies, work in C. elegans has also identified surprising cell-non-autonomous mechanisms that contribute to cellular protein folding in an organismal context (Garcia et al., 2007; Prahlad et al., 2008; Prahlad and Morimoto, 2011).
The basis for an EFD system selection includes four main subsystems (Access, Drill Site, Rig, and Operation) and 13 subsets, which have been previously identified through standard EFD operations (see Figure 1).
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This gene was previously identified through analyses of proteins unique to each X. bovienii strain [ 5].
FABP2 p.A55T, which was previously identified through sequencing [ 18], was genotyped in this work using the restriction enzyme Hha I.
These included ISADAMMQALLGAR, SSANYR, ISASR, ALSTR, ALSTR, and FKRPTLR, which have all be previously identified through studies using P labeling and two-dimensional peptide mapping.
Five as1 (formerly known as bibb) mutants were previously identified through a genetic screen for organ shedding mutants [ 12] (Additional file 1: Table S1); all mutants are of the Landsberg erecta (L er) ecotype.
FRAP1 (mTOR) (mean SI = 0.212; Table 1) was previously identified through an RNAi screen as a target of paclitaxel sensitivity, and was used in our screen as a positive control in each plate [ 30].
The aim of this study was to evaluate the mutation pressure on the non-syntenic blocks (NSBs) of the genome, which were previously identified through comparative genomic analysis of A. oryzae, Aspergillus fumigatus, and Aspergillus nidulans.
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