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Furthermore, our TMD models were used to study the effects of previously reported GPHR TMD mutations on the molecular interactions and activity of the GPHRs which had not been previously explained at the structural level.
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Wave-enhanced interfacial transport by small-amplitude waves at low flow rates has been previously explained as distortion of the diffusion boundary layer by the normal disturbance velocity.
Increasing ED attendance has been previously explained as a consequence of free service and treatment.
Flow cytometric approaches have been previously explained in detail.
For example, related photographs appear when the landmark photograph is clicked as was previously explained.
This is why a hierarchical validation is used, as previously explained.
Human normal ERYs were obtained as previously explained by plating CD34 HPCs at a density of 0.5 × 10 cells/ml in IMDM supplemented with 20% BIT StemCell Technologiess), 50 ng/ml SCF and 4 U/ml EPO (R&D Systems).
Duplication of chromosome 18 and loss of 8p has been previously reported in PC346Flu2 and PC346DCC, respectively, and may explain the clustering observed at these loci [20].
Therefore, some of these partitions may be incorrect, as previously explained.
We and others, have previously observed a similar effect in Dync1h1Loa/+;SOdouble double heterozygotes although this finding has not yet been explained at a molecular level [20], [21], [23].
These findings support our previous hypothesis, based on physiological parameters, of how N starvation affects the cell function since the previously described effects can be explained at a biochemical level based on PC1 and PC2 (plotting other components does not improve the separation between groups (Additional file 8: Figure S7d, e, f)).
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