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Bbs8-shRNA has been previously design and validated in (12) and the same transfection protocol was followed.
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For cutting force measurement, a test setup that was previously designed and constructed for cutting force measurement was used.
The C34 trimeric molecule was previously designed and synthesized using a novel template with C3-symmetric linkers by us.
These questions were previously designed and pilot-tested in COPD patients from the same geographical area [ 18].
Primers for sugarcane SSRs derived from expressed sequence tags (ESTs) and intergenic sequences were previously designed and characterized by James et al., 2011 [ 29].
bThese primers were previously designed and used in Rise et al. [ 25] but were still quality checked for the present experiment using the reference cDNA as template.
The primer sequences, amplification efficiencies and amplicon sizes are shown in Table 3. aThese primers were previously designed and used in Rise et al. [ 22] but were still quality checked for the present experiment using the reference cDNA as template.
Record linkage was performed using an upgraded version of an ad hoc software application that was previously designed and validated in Italy and Switzerland (Geneva) to match individuals from AIDS and cancer registries while protecting patient anonymity (Dal Maso et al, 2001; Clifford et al, 2005).
The RCAHEST can be used for examining the seismic performance of structures that have been previously designed.
He was previously design master at Samsung C&T, its engineering and construction group.
The questionnaire was previously designed, planned and piloted by the Diabetes Care Team in collaboration with patients at George Eliot Hospital NHS Trust, Nuneaton, Warwickshire, UK. Results on the 10 cm VAS were converted into an equivalent numerical value between 0 to 100, to the nearest whole figure.
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