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As has been previously described, using more than one generic primer set allowed us to detect a greater number of infected women [24], as well as a better coinfection estimate [25].
All of the above described modifications have been previously described using different labeling strategies and methods to characterize in vitro and in vivo biological properties.
The early taste buds on the zebrafish larval head (3 5 dpf) and on the budding maxillary barbel have been previously described using light, scanning and transmission electron microscopy [31].
This lead to a computational recapitulation of a known mechanism for integrin ITB3 activation and signaling (which has been previously described using in vitro methods [58], [59], [60], [61], [62], [63], [64]) as follows: Threonine phosphorylation of ITB3 maintains it in the inactive state in resting platelets.
The lack of potentiating effects cannot be attributed to the use of an amphetamine dose producing "ceiling" functional effects, as region-specific potentiation of response by different pharmacological mechanisms has been previously described using the same protocol and dose of D-amphetamine [18], [20].
Edema after deployment of RF lesion has been previously described using intracardiac echocardiography.
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Interestingly, an ortholog for the chaperonin GroEL was not found, however this protein was previously described using 2D gel electhrophoresis and MALDI-TOF analysis of M. suis [33].
Detection of lymphatic metastases was previously described using oncolytic viruses carrying eGFP [45], [52], [56] or Renilla luciferase reporter genes [56].
The kinetics of MeHg in rats was previously described using a detailed PBPK model by Carrier et al. (2001).
Thalamic volume loss was previously described using cross-section diameter measures on MRI scans, 21 but not yet utilizing volumetric analysis.
Health status and health-related quality of life (HRQoL) in RVVC were never previously described using validated questionnaires.
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