Sentence examples for been previously confirmed using from inspiring English sources
The specificity of the PCR assay has been previously confirmed using pure isolates of closely related bacterial species, including B. pseudomallei, B. thailandensis and B. cepacia.
There are canonical targets described for these miRNAs and the cleavage of these targets by their respective miRNAs was previously confirmed using RACE 5′ PCR [ 60– 60].
The polygenic architecture of gF and gC was previously confirmed using the Cognitive Aging Genetics in England and Scotland (CAGES) cohort as the discovery sample and the Norwegian Cognitive NeuroGenetics (NCNG) adult lifespan sample for replication (Davies et al. 2011).
Quantitative accuracy of Zr PET scans was previously confirmed using phantom studies and comparisons between PET-derived and sampled blood radioactivity concentrations, as well as between PET-derived and biopsy tumour radioactivity concentrations [ 17].
Dehiscence of the left superior canal had been previously confirmed in this patient using high-resolution CT imaging of the temporal bone and VEMP testing.
The outbreak strain's antibiotic resistance plasmid was previously confirmed as IncI1 ST31 using traditional plasmid MLST (present in the IncI1 database as CTX-I1-O104 CTX-I1-O104 CTX-I1-O104org/plasmid/).
The isolates used in the present study were previously confirmed as P. aeruginosa using growth morphology, Gram stain, and API test strips, and their type three secretion gene profile was determined [ 29].
The presence of M. leprae DNA in the Sk14 extract was previously confirmed by real-time PCR using primers for the multicopy RLEP and the single copy locus, ML1795 (encoding the 18 kDa antigen), both of which gave single bands of the expected size (111 bp and 114 bp, respectively) [ 13].
The three assays were further evaluated for detection capability using 21 archived ERAV (n = 11) or ERBV (n = 10) isolates whose identity was previously confirmed in a one-way neutralization test using mono-specific rabbit antisera [ 16].
Developed assays were tested using norovirus positive stool samples which were previously confirmed to present target genotypes of this study.
DOI: http://dx.doi.org/10.7554/eLife.08827.017 10.7554/eLiFigure27 figuregure 7—figure supplement 2. Morpholino effects seen in this paper that have not previously been validated were confirmed using a second morpholino designed in a location distinct to the site of the first morpholino.
