Following Yerokhin [14], in order to perform the anodizing process, Ta sample surfaces were previously cleaned in ethanol and distilled water and then air-jet dried.
All PTFE digestion vessels were previously cleaned in a bath of 10% (v/v) nitric solution for 48 h to avoid cross-contamination.
SSL application – Top Electrode Lamination Layer: 250 nm Au was deposited using an e-beam evaporator onto a bare Si wafers (University Wafers, Boston, MA) that was previously cleaned in a solution of H2SO4 + H2O2 for 15 min and rinsed with DI water.
Films were spin-coated onto Spectrosil quartz substrates that were previously cleaned via successive sonication in acetone and isopropyl alcohol.
Make sure all equipment has been previously cleaned, rinsed with deionized water, and allowed to dry.
The coupons were previously cleaned by immersion in a 70% ethanol solution, and then aseptically and individually washed with ultra-pure sterile water and let to dry overnight at room temperature (21°C).
The liver and the intestine which was previously cleaned up and weighed, were digested separately in 4 % KOH solution at 37 °C for 6 h.
As the direct transmission was studied in previously cleaned and disinfected facilities the pathogen load was probably low.
The samples were collected in previously cleaned polyethylene bottle of capacity 1000 ml and mixed with appropriate preservatives for further analysis.
