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Because darting during fear conditioning has not been previously characterized, we chose to set a relatively inclusive criterion.
Because H2AB has not been previously characterized, we used a molecular evolution approach to investigate its relationship to H2A variants that have been characterized more thoroughly.
As the evolvability of this category of residues has not been previously characterized, we excluded them from the reference set, which finally was formed only by those mtDNA-encoded residues exposed at the protein surface that are not involved in any sort of intersubunit contacts.
Because some aspects of the function of Fox2 and its binding sites within RNA sequences have been previously characterized, we were able to predict the effect of variation of Fox2 mRNA expression on a subset of exons that contain its putative binding sites.
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Since AmphiVent2 has not been previously characterized at all we performed an expression analysis using real-time quantitative RT-PCR.
As many glucocorticoid- and LPS-responsive genes have been previously characterized by us and others [ 20– 22], we selected for validation either poorly characterized or novel target genes.
As several LPS-induced, Dex-repressed genes coding for various cytokines that we found in clusters 3 (Tnf, Ccl2, 3 and 4), 4 (Il1andnd b) and 5 (Lif, Niacr1, Mmp13) have been previously characterized by us and others [ 20– 22], we focused on uncharacterized LPS/Dex targets.
We tested several of the isolates that had been previously characterized.
This error has not been previously characterized.
Although mammalian TRICs are known as K+ channels23,29,30,33, the function of prokaryotic TRIC had not been previously characterized.
The localized electromagnetic correlates of basic phrase building have been previously characterized for spoken language, both in comprehension9 and production8.
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