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Although the copper-regulated tcu-1 has been previously characterized, this is the first investigation exploring nitrogen-controlled nit-6 for expression of heterologous genes in N. crassa.
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As detailed below, although breast tumours with an aggressive phenotype that express these cytokeratins were previously characterized, this subtype of tumour gained considerable notoriety with their rediscovery by the use of cDNA microarrays.
Zebrafish IFN and IFN receptors have been previously characterized, and this manuscript uses the IFNΦ nomenclature suggested by the authors (Levraud et al., 2007; Aggad et al., 2009).
The allozyme loci GOT1 and GOT2 were previously shown to be linked [ 31], lending further credence to the idea that the GOT1p1 and/or GOT1p2 loci might encode the allozyme marker GOT1 that has been previously characterized in this species.
They reported 30 full conopeptides precursor sequences (i.e. those beginning with a methionine residue and finishing by a stop codon) that had been previously characterized in this species.
We disregarded the study of defence-related genes encoding PR1, oxidoreductase, and chlorophyll a/b binding protein, whose role and/or behavior has been previously characterized for infections with this pathogen [ 36, 37].
Although urinary gonadotropins have been previously characterized as safe [19], [20], this opinion may be overly optimistic in view of the present findings, supported by results from other recently published studies.
A number of genes have been previously characterized to be involved during this transition.
Although tcu-1 has been previously characterized (Lamb et al. 2013), this is the first implementation of nit-6 as a regulated promoter in N. crassa.
E. coli strains used in this study had been previously characterized for the virulence genotype [9].
This plasmid has been previously characterized and confers a nonmating (diploid) phenotype in haploid BY4741 and BY4742 cells [41].
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