Seventy-two sequences were characterized for this study, the bulk of which are newly-characterized (47 sequences) or were previously characterized from other strains (14 sequences), were available as ESTs in public databases (1 sequence) or are previously published and confirmed here (10 sequences; see Additional file 1).
Since MWCNTs purification and functionalization is a key point for the electroanalytical performance of the final devices, MWCNTs were previously characterized from the morphological, surface and electrochemical point of view.
IFN alpha and IFN beta have been previously characterized by cloning from stimulated cells [ 40].
As the evolvability of this category of residues has not been previously characterized, we excluded them from the reference set, which finally was formed only by those mtDNA-encoded residues exposed at the protein surface that are not involved in any sort of intersubunit contacts.
Recognition sites for only a few BRDs have been previously characterized, and reported substrate affinities range from the low micromolar to the millimolar KD range.
Total of eight CC cell lines (HeLa, SiHa, ME-180, C-33A, CaSki, C-4I, MS751, and SW756) that have been previously characterized [ 24– 27] were kind gift from Dr. V. V. S. Murty, Columbia University, New York, USA.
