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Sample selection was based on HCV genotype as had been previously assigned using the LiPA.
Sample selection was based on HCV genotype as had been previously assigned using the Versant™ HCV genotype Assay-LiPA (version I; Siemens Medical Solutions, Diagnostics Division, Fernwald, Germany).
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All of these populations had been previously assigned to group2.
For example, bands in the BR spectrum were previously assigned to an all- trans-retinal chromophore using model retinal compounds and isotope labeling.C solid state NMR also provides strong confirmation of the existence of an almost pure all- trans configuration of the retinal chromophore in the case of BR.
Isolate EU332330 from China was previously assigned to PAV-III subspecies, whereas isolate EF521850 was assigned to PAV-I.
LSTc and LSTa resonances were previously assigned.
Amongst the latter previously deposited sequences, a total of 54 putative HRV-C (geno types could be assigned using previously described criteria (VP4/VP2 gene phylogeny and a pairwise distance threshold of 0.1; [18]).
(2) Metabolite signals in ITOCSY spectra were assigned using previously established methods.
The linkage groups were assigned using previously mapped loci to ensure consistency with the published integrated map of lettuce (Truco et al. 2007).
The chromosomal patterns were examined visually and similarities of the PFGE patterns were assigned using criteria published previously [ 26].
Radiation skin damage scores were assigned using a murine skin radiation damage grading scale slightly modified from a previously published scale [ 36, 37].
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