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We injected intradermally in nude mice gelatine spheres that had either been preseeded with human fibroblasts or preadipocytes, or left unseeded.
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The cells were plated in 15 cm Petri dishes (1000 cells per dish), which had been preseeded with 10 lethally irradiated (40 Gy) mouse 3T6 fibroblasts.
Once the inner surface of a bilayer NGC has been preseeded with Schwann cells, it should be able to effectively guide the extension of neurites during regeneration, similar to the conduit based on a single layer of aligned nanofibers.
When the scaffold was preseeded with Schwann cells, more neurites were observed to grow from the cell body within the same culture time.
Both histomorphometric analysis and functional characterization demonstrated that bilayer NGCs with an inner surface that was preseeded with Schwann cells could reach 54%, 64.2%, and 74.9% of the performance of isografts in terms of nerve fiber number, maximum isometric tetanic force, and mass of the extensor digitorum longus muscle, respectively.
Microtitre plates (96 well) were obtained preseeded with V79 cells at 10 000 cells ml−1 (European Collection of Animal Cell Cultures, ECACC) in DMEM+10% FCS.
"Here, they are leading with human capital".
"It is rampant with human rights violations".
The problem is addressed with human locomotion as a reference.
The reliability of sensor was evaluated with human urine samples.
The complete tissue-engineered (TE) bone was constructed of the material preseeded with allogeneic bone marrow mesenchymal stem cells (MSCs).
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