Sentence examples for been prepared from single from inspiring English sources

Compact-type (CT) fatigue crack growth (FCG) specimens have been prepared from single crystals of SRR99.

FAMEs standard mixture (C12 - C22) was prepared from single standard purchased from Grace (USA) and Supelco Inc. (Bellefonte, PA, USA).

The pre-cultures were prepared from single colonies at 30°C and 150 rounds per minute (rpm) in 200 mL of the same medium with fructose as the sole carbon source under aerobic conditions.

Precultures were prepared from single colonies in 3 mL over night at 30 °C, larger cultures were inoculated to an initial OD 600) of 0.1, and cultivated in 20 mL medium (100 mL unbaffled shake flasks) at 30 °C and 200 rpm orbital shaking.

Working stocks were prepared from single individual colonies exhibiting the large-light phenotype [47].

Peripheral blood mononuclear cells were prepared from single donor leukocyte buffy coats (National Blood Service) and T cells expanded as previously described and used between days 10 and 14 [214.

Human T lymphocytes were prepared from single donor peripheral blood mononuclear leukocytes by treatment with 0.5% phytohemagglutinin (PHA, Sigma, Gillingham, UK) for 48 h.

CD8+ T cells from Clone 4 TCR Thy1.1 transgenic mice and CD4+ T cells from HNT Thy1.1 were prepared from single cell LN suspensions by magnetic depletion using the T cell isolation kits and an AutoMACS apparatus from Miltenyi according to the manufacturer's instructions.

cDNA was prepared from single cells by direct addition of the crude lysate to the Super SMART™ PCR cDNA amplification kit (Clontech, BD) followed by first-strand cDNA synthesis and aRNA amplification for 40 cycles, performed according to the manufacturers instructions.

DNA was prepared from single nematodes as described in [ 48].

Genomic DNA was prepared from single male mosquitoes from three laboratory strains; G3, L3-5 and Yaounde.