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Author: Burrows, D.; Alexander, G. Background This paper has been prepared as input into the preparation of UNICEF's regional strategy for HIV prevention in the CEE/CIS and Central Asia.
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Be prepared as soon as possible.
A serial dilution of DNA from input and immunoprecipitated DNA for the Slot Blot assay were prepared as follows: For input DNA, 100 μl denatured DNA; 50 μl denatured DNA + 50 μl H2O; 25 μl denatured DNA + 75 μl H2O.
The S. cerevisiae formaldehyde cross-linked sheared input samples were prepared as described [28].
An Illumina library of affinity-purified and input DNA were prepared as previously described [ 26].
PCR mixes were prepared as follows: 5 μL of cDNA calculated on input total RNA for each individual gene was added to 20 μL of PCRMix (ABgene, UK).
Cultured granule neurons were treated with etoposide and eTIP fractions (IP input, IP, P1, and P2) were prepared as described in the eTIP procedure.
Chromatin from IMR-32 cells was prepared as manufacturer's instruction ChIP-IT Expresss, Active Motif); a little portion of the supernatants was kept as "input" material (0,5% total chromatin).
were prepared as described [38].
dsDNA templates were prepared as described [30].
Protein samples were prepared as described [22].
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