Given the evidence for PpIX accumulation in palpable, mature mammary tumours, we administered 5-ALA to WaptTag1 transgenic female mice prior to the anticipated age for palpable tumour presentation, when early stages of tumour progression are predicted for this mouse model.
A similar number of sites was predicted for the orthologous mouse, human and rhesus macaque sequences.
Transcription factor binding sites (TFBS) were predicted for murine (NCBI mouse genome version 33) promoter regions (-5000 to +500 bp with respect to the transcription start site) on a genome-wide scale using Genedata Phylosopher® version 6.5 Promoter Explorer (PE).
Further downstream, CpG islands are predicted for both human and mouse (figure 16), 100 200 bp upstream of the GenBank TSS.
The PFAM protein domains (PfamA-27.0) (Finn et al., 2014) were predicted for all those proteins from human, mouse, fly and mosquito and the planarian homologs of solute carriers using the InterProScan (version 5.4 47.0) tool (Jones et al., 2014).
These studies indicate that the ability of bimatoprost to stimulate hair growth may extend beyond eyelashes, but effects on human scalp hair growth, for example, cannot necessarily be predicted from mouse pelage hair experiments.
Three PNPLA2 protein isoforms, whose sizes were consistent with those predicted for mouse in UniProt (UniProt Consortium, 2014), were detected by Western blot.
