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In 2014, plugs had been preconditioned with Titanoderma sp. for over six months and were covered with the coralline algae.
The eluate was trapped on a cation exchange cartridge (BondElut SCX, Varian) which had been preconditioned with 5.5 M HCl.
Membranes were then aspired onto a glass fiber filtermat (Printed Filtermat B, Wallach, Turku, Finland), which had been preconditioned with 0.33% polyethylenimine for 30 min and then washed five times with 300 μL (per well) of wash buffer (50 mM HEPES, 500 mM NaCl, 0.1% BSA, buffered to pH 7.4 using 1 N NaOH), using a Mach II M Harvester 96 (Tomtec CE, Etten-Leur, The Netherlands).
This sample was applied to RP-18 solid-phase extraction columns (100 mg) that had been preconditioned with 1 mL of acetone followed by 1 mL of 15% (vol/vol) methanol in 0.1% (vol/vol) formic acid.
The dissolved solution was passed through an Oasis hydrophilic-lipophilic balance solid phase extraction cartridge (2 g/12 mL; Waters, Milford, MA, USA) that had been preconditioned with 10 mL 100% methanol (MeOH) and 10 mL deionized water.
Purified [F]FBABM from the previous step (∼12 mL) was diluted with water (∼50 mL) and passed through a Waters C18 SepPak that had been preconditioned with 5 mL ethanol and 5 mL water.
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The rat brain was preconditioned with 1 U thrombin by direct infusion into the left caudate nucleus.
Interventions: The IP patients were preconditioned with 2 periods of 2-minute ischemia followed by 3-minute reperfusion before aortic cross-clamping.
Next, rMSCs in each scaffold were preconditioned with chondrogenic media in vitro for 1 week and implanted in the backs of nude mice for 6 weeks.
By inverting these graph Laplacian matrices, the discretized single layer operator is obtained, which can be preconditioned with the hierarchical basis.
The results may provide a mechanistic explanation for the improved outcome to polymicrobial sepsis of rats that are preconditioned with heat shock, as well as a novel highlight for therapeutic intervention of severe infection.
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