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Since the release of the Infinium HumanMethylation450 array (Illumina, Inc). in 2011, it has been possible to interrogate almost half a million CpG sites in the human genome at less cost than with next-generation sequencing (NGS).
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It was shown that it is possible to interrogate and observe muscle behavior in real time, as well as selectively stimulate the contraction of myotubes with the device.
Thus, it is possible to interrogate all 1024 unique pentamer binding sites with a single microarray.
Once genomic coordinates and alleles are known (from sequencing), it is possible to interrogate these variants using standard genotyping arrays.
It will be possible to interrogate the data to identify whether individuals accumulate 30 min of moderate activity a day.
Using GREAT [ 34], it was possible to interrogate annotations from 20 different ontologies utilising the genomic coordinates of the DMRs.
Using SMRT sequencing it is possible to interrogate the native methylome of the genome as no amplification steps occur prior to sequencing.
As such, the skin is an excellent model system for these studies; it is accessible, it has a relevant environmental carcinogen (UVB), and it is possible to easily interrogate human disease.
Since the NP film PNR fabrication process results in such a uniform population of PNRs, it is possible to simultaneously interrogate an ensemble of PNRs with a single optical measurement, as opposed to having to identify and characterize the rulers one-by-one, as is the case for immobilized NP dimer PNRs.
Once a region of interest has been identified and the haplotypic status of the sequenced affected and unaffected individuals ascertained, it may be possible to further interrogate variants under certain genetic models.
Unfortunately, unlike studies of human and some animals, it is not possible to interrogate plants directly; answers relating to cognition and memory, for example, can be received only indirectly through behavioral responses and, then, be comprehended only in the light of human knowledge systems, personal subjective experience, and degree of understanding.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com